Exam Code: MCAT
Exam Questions: 815
Medical College Admission Test: Verbal Reasoning, Biological Sciences, Physical Sciences, Writing Sample
Updated: 25 Nov, 2025
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Question 1

In an acetyl molecule, two atoms of carbon are bonded by: 


Section: Physical Sciences 

Options :
Answer: D

Question 2

Our sense of smell is arguably the most powerful of our five senses, but it also the most elusive. It plays a vital
yet mysterious role in our lives. Olfaction is rooted in the same part of the brain that regulates such essential
functions as body metabolism, reaction to stress, and appetite. But smell relates to more than physiological
function: its sensations are intimately tied to memory, emotion, and sexual desire. Smell seems to lie
somewhere beyond the realm of conscious thought, where, intertwined with emotion and experience, it shapes
both our conscious and unconscious lives.
The peculiar intimacy of this sense may be related to certain anatomical features. Smell reaches the brain more
directly than do sensations of touch, sight, or sound. When we inhale a particular odor, air containing volatile
odiferous molecules is warmed and humidified as it flows over specialized bones in the nose called turbinates.
As odor molecules land on the olfactory nerves, these nerves fire a message to the brain. Thus olfactory
neurons render a direct path between the stimulus provided by the outside environment and the brain, allowing
us to rapidly perceive odors ranging from alluring fragrances to noisome fumes.
Certain scents, such as jasmine, are almost universally appealing, while others, like hydrogen sulfide (which
emits a stench reminiscent of rotten eggs), are usually considered repellent, but most odors evoke different
reactions from person to person, sometimes triggering strong emotional states or resurrecting seemingly
forgotten memories. Scientists surmise that the reason why we have highly personal associations with smells is
related to the proximity of the olfactory and emotional centers of our brain. Although the precise connection
between emotion and olfaction remains a mystery, it is clear that emotion, memory, and smell are all rooted in a
part of the brain called the limbic lobe.
Even though we are not always conscious of the presence of odors, and are often unable to either articulate or
remember their unique characteristics, our brains always register their existence. In fact, such a large amount
of human brain tissue is devoted to smell that scientists surmise the role of this sense must be profound.
Moreover, neurobiological research suggests that smell must have an important function because olfactory
neurons can regenerate themselves, unlike most other nerve cells. The importance of this sense is further
supported by the fact that animals experimentally denied the olfactory sense do not develop full and normal
brain function.
The significance of olfaction is much clearer in animals than in human beings. Animal behavior is strongly
influenced by pheromones, which are odors that induce psychological or behavioral changes and often provide
a means of communicating within a species. These chemical messages, often a complex blend of compounds,
are of vital importance to the insect world. Honeybees, for example, organize their societies through odor: the
queen bee exudes an odor that both inhibits worker bees from laying eggs and draws drones to her when she
is ready to mate. Mammals are also guided by their sense of smell. Through odors emitted by urine and scent
glands, many animals maintain their territories, identify one another, signal alarm, and attract mates.
Although our olfactory acuity can’t rival that of other animal species, human beings are also guided by smell.
Before the advent of sophisticated laboratory techniques, physicians depended on their noses to help diagnose
illness. A century ago, it was common medical knowledge that certain bacterial infections carry the musty odor
of wine, that typhoid smells like baking bread, and that yellow fever smells like meat. While medical science has
moved away from such subjective diagnostic methods, in everyday life we continue to rely on our sense of
small, knowingly or not, to guide us.
It can be inferred from the passage that the emotional element of human olfaction would be better understood
through investigation into:

Section: Verbal Reasoning 

Options :
Answer: A

Question 3

A researcher in a molecular biology lab planned to carry out an extraction procedure known as an alkaline
plasmid prep, which is designed to purify plasmids, small pieces of the hereditary material DNA, from bacterial
cells. The bacteria are first placed into a test tube containing liquid nutrient medium and allowed to grow until
they reach a high population density. The culture, which consists of solid cells suspended in the medium, is
then centrifuged; a solid pellet is formed. The supernatant is poured out, leaving the pellet behind, and the cells
are resuspended in a mL of lysis buffer solution (50 mM glucose, 25 mM Tris buffer and 10 mM
ethylenediaminetetraacetic acid (EDTA), with 5 mg of the enzyme lysozyme added). They are then incubated
for 30 minutes at 0° C, during which time the bacterial cell walls break down and the cell contents are released
into the solution. After incubation, 1 mL of 0.4 N sodium hydroxide and 1 mL of 2% sodium dodecyl sulfate
(SDS) are added, and the solution is again incubated on ice for 10 minutes. 2 mL of 3 M sodium acetate are
added and the mixture is incubated for 30 minutes at 0° C. The test tube is centrifuged once more and the
supernatant is decanted into a clean tube, leaving behind the protein and most other cell components in the
pellet.
Finally, 10 mL of pure ethanol are added to the supernatant from the previous step to precipitate out the DNA,
and the test tube is incubated at −20° C for 60 minutes, during which the mixture remains liquid. The mixture is
centrifuged a final time and the supernatant removed. The translucent precipitate that results is washed with
70% ethanol (70% ethanol and 30% water by volume), allowed to dry, and resuspended in 1 mL of TE buffer
(10 mM Tris, 1 mM EDTA).
In preparation for this experiment, the researcher prepared stock solutions of the various chemicals that she will
need in the experiment. Stock solutions are highly concentrated solutions of commonly used chemicals in water
from which dilute solutions are prepared for daily use. Table 1 shows the chemicals, their molecular formulas
and weights, and the composition of commonly used stock solutions.
MCAT-part-3-page300-image175
Which of the following conclusions can be reached based on the fact that DNA precipitates in the last step of
the plasmid prep procedure?

Section: Physical Sciences 

Options :
Answer: B

Question 4

Four major blood types exist in the human ABO blood system: types A, B, AB, and O; and there are three
alleles that code for them. The A and B alleles are codominant, and the O allele is recessive. Blood types are
derived from the presence of specific polysaccharide antigens that lie on the outer surface of the red blood cell
membrane. The A allele codes for the production of the A antigen; the B allele codes for the production of the B
antigen; the O allele does not code for any antigen.
While there are many other antigens found on red blood cell membranes, the second most important antigen is
the Rh antigen. Rh is an autosomally dominant trait coded for by 2 alleles. If this antigen is present, an
individual is Rh+; if it is absent, an individual is Rh−. For example, a person with type AB blood with the Rh
antigen is said to be AB+.
These antigens become most important when an individual comes into contact with foreign blood. Because of
the presence of naturally occurring substances that closely mimic the A and B antigens, individuals who do not
have these antigens on their red blood cells will form antibodies against them. This is inconsequential until
situations such as blood transfusion, organ transplant, or pregnancy occur.
Erythroblastosis fetalis is a condition in which the red blood cells of an Rh+ fetus are attached by antibodies
produced by its Rh− mother. Unlike ABO incompatibility, in which there are naturally occurring antibodies to
foreign antigens, the Rh system requires prior sensitization to the Rh antigen before antibodies are produced.
This sensitization usually occurs during the delivery of an Rh+ baby. So while the first baby will not be harmed,
any further Rh+ fetuses are at risk.
The Coombs tests provide a method for determining whether a mother has mounted an immune response
again her baby’s blood. The tests are based on whether or not agglutination occurs when Coombs reagent is
added to a sample. Coombs reagent contains antibodies against the anti-Rh antibodies produced by the
mother. The indirect Coombs test takes the mother’s serum, which contains her antibodies but no red blood
cells, and mixes it with Rh+ red blood cells. Coombs reagent is then added. If agglutination occurs, the test is
positive, and the mother must be producing anti-Rh antibodies. The direct Coombs test mixes the baby’s red
blood cells with Coombs reagent. If agglutination occurs, the test is positive, and the baby’s red blood cells
must have been attacked by its mother’s anti-Rh antibodies.
Based on information in the passage, what does the reaction below represent?


Section: Biological Sciences 

Options :
Answer: B

Question 5

For the last two decades many earth scientists have supported the notion that the Mediterranean was once a
huge, dry desert, lying 3,000 meters below sea level. This “death valley” was thought to have existed at the end
of Miocene time, about 6 to 5.5 million years ago…
…From a geological point of view, the Mediterranean is a tectonically mobile land-enclosed depression – small
(about 3,000,000 square kilometers) in comparison to the major world oceans…Immediately obvious on all
charts is the highly variable topography and relief of both the seafloor and adjacent borderland. The coastline is
highly irregular and continental shelves, though generally narrow, are well developed off the major river deltas
(Nile, Rhone, Po, and Ebro). Moreover, the deep-sea basins and trenches have distinctive relief, with basin
plains ranging in depth from less than 1,000 meters to more than 4,000…Observation that rocks dredged
offshore are similar to those on land raised a fundamental concept – the key to understanding Mediterranean
history lies in the adjacent emerged land masses, and vice-versa…
…Early paleographic reconstructions showed that the once-open communication with the Atlantic deteriorated
during the upper Miocene. Water-mass exchange continued for a while in the Rif Strait, but then ceased
completely prior to the beginning of the Pliocene…
…High relief near what is now the Strait of Gibraltar served as a barrier to the exchange of waters with the
Atlantic. Exposed to a hot and dry climate, water evaporated and the then-dry basin elicited comparison with a
gigantic Death Valley…Microfossil studies suggested that the depth of the Mediterranean basin at these times
had been “deep.” Estimates suggested a dry seafloor as far as 2,000 meters below ocean level… As a
response to suddenly lowered sea level, rivers feeding the Mediterranean and canyons on the now-dry seafloor
began a geologically dramatic phase of erosion. Deep, Grand Canyon-like gorges of the Nile and Rhone rivers,
presently buried on land, were apparently cut during a great drawdown of water – when the Mediterranean floor
lay exposed 1,000 meters or more below its present level…The sudden flooding through a gigantic waterfall at
Gibraltar drowned the exposed basin floor. These falls would have been 1,000 times bigger than Niagara
Falls…This flooding event is recorded by the Miocene Pliocene boundary, a time when open marine faunal
assemblages were suddenly reintroduced from the Atlantic…
…Geological theories usually fall at a glacial pace into a sea of controversy, and this one is no exception. Today
– charging that proof for the theory is lacking – many scientists believe that the Med always contained saltwater,
with only the depth of the seafloor and the water being in question… Some of the tenets on which the theory
was formulated are, if not defective, very seriously in question. To interpret their findings, a respectable number
of geologists studying the surrounding emerged borderland as well as subsea sections indicate that alternative,
more comprehensive concepts must be envisioned…
…It is not realistic to envision the Mediterranean seafloor of about 5 million years ago as a desert at 3,000
meters below present ocean level. Several years ago…the Mediterranean [was compared] to a complex
picture-puzzle that comprises numerous intricate pieces, many of which are already in place. A general image
is emerging, although gaps in some areas of the picture remain fuzzy and indistinct.
All of the following are features of the “desert theory” EXCEPT:

Options :
Answer: A

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